Coding
Part:BBa_K302029:Design
Designed by: Philip Hall Group: iGEM10_Newcastle (2010-10-04)
sacB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sacB sequence encodes for levansucrase enzyme of Bacillus Subtilis. The gene has been modified by removing an ecoRI at position 201 using Bacillus Subtilis codon bias and by changing the stop codon for the recommended double TAA.